ntration. Curcumin feeding of the double inserted Ab142 expressing flies showed a positive effect at low and intermediate concentrations of curcumin treatments. The lifespan for high curcumin concentration treated flies was not apparently different when compared to the untreated flies. All curcumin treatments of the Ab142 E22G expressing flies showed a substantial positive effect upon curcumin treatment. The greatest observed effect was found on 0.001% curcumin 1032568-63-0 treatment of the Ab142 E22G expressing flies, which increased the T1/2 by 75% compared to untreated flies. Curcumin feeding of the Tau expressing flies rendered no effect on survival at low concentrations, but a toxic effect on high concentrations of curcumin. The median survival time of all transgenes and curcumin concentrations displayed a clear effect upon curcumin treatment for genotypes having the strongest phenotype. The toxic effect on curcumin treatment was balanced with the rescuing effect of genotypes having a mild phenotype. The T1/2 for all genotypes and curcumin concentrations are summarized in 24 hours. This setup allowed for characterization of the locomotor and behavior rhythms of Drosophila. Comparing the locomotor activity of the flies without curcumin treatment at day 5, there were obvious differences in the activity and circadian rhythm between the different genotypes. The DAM2 system hence appeared more sensitive for assaying early signs of neurological impairment compared to the conventional climbing assay. Studies of continuous curcumin treatment of flies at the intermediate concentration 19071018” was performed at different days of aging, with 5 days increments. Control flies showed slight activity deterioration upon curcumin treatment. Overall, the locomotor activity of the flies decreased with increasing age, but the number of beam breaks per hour was almost consistent during the first hours of the assay. The decreased total number of beam brakes upon increased age was caused by the shortened number of active hours. Ab140 expressing flies showed no activity improvement upon curcumin treatment. Single insert Ab142 expressing flies showed a higher number of beam breaks and a continuation of activity during a larger number of hours upon curcumin treatment for flies at 5 and 10 days. The effect of curcumin showed a tendency of decreasing with age. Double insert Ab142 expressing flies showed an activity enhancement upon curcumin treatment for flies of all ages. Also here the effect of curcumin showed a tendency of declining with age. The Ab142 E22G expressing flies showed a severely decreased locomotor activity already at day 5 compared to control flies. Curcumin treated Ab142 E22G expressing flies at day 5 showed an increased number of beam breaks per hour during the first hours, but the number of active hours was not significantly enhanced. A small but significant increase in activity 
was observed for 10 days old Ab142 E22G expressing flies treated with curcumin. The activity assay was performed with the unusual population of the Ab142 E22G expressing flies surviving two days longer than T1/2 of the untreated Ab142 E22G expressing flies. No activity assay was performed for Ab142 E22G expressing flies at ages beyond 10 days, due to their short lifespan. Tau expressing flies appear severely affected in their locomotor activity, and interestingly showed a large enhanced activity during the first hours upon curcumin treatment. The activity enhancement was sustained with
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