hibited the upregulation ofFrontiers in Plant Science | frontiersin.orgAugust 2021 | Volume 12 | ArticleTorres-Silva

hibited the upregulation ofFrontiers in Plant Science | frontiersin.orgAugust 2021 | Volume 12 | ArticleTorres-Silva et al.De novo Transcriptome of M. glaucescens Shoot Organogenesisseveral transcription variables associated together with the biosynthesis of secondary metabolites (i.e., bHLH, MYB, NAC, WRKY, and YABBY) (Table three) (Pal et al., 2018; Kayani et al., 2019; Xie et al., 2020). The biosynthetic pathways activated in the course of shoot organogenesis induction of M. glaucescens indicate a sturdy prospective for the production of bioactive compounds having a wide array of functions. Although the present findings could enable optimize the micropropagation of M. glaucescens for horticultural and ornamental purposes and facilitate the conservation of endangered species along with the biosynthesis of beneficial compounds, the results of this study are determined by seed propagation. Consequently, a number of the observed variations in organogenesis could be a result of genetic variability across samples. A earlier study (Torres-Silva et al., 2018) has shown that clonal propagation is also correlated with somaclonal variation in Melocactus; hence, genetic variability within sexually or asexually generated populations could nevertheless be a confounding variable.AUTHOR CONTRIBUTIONSGT-S, AK, DB, SR, SS, CS, and WO conceived and created the study. GT-S Topo II web performed the experiments. GT-S and AK performed shoot organogenesis induction, sample collection, and RNA extraction. LC and DB performed RT-qPCR analyses. GT-S and SS performed de novo assembly pipelines. GT-S, SS, AA, and CS performed bioinformatics analyses. GT-S, DB, AK, SR, SS, AA, ER, DC, CS, and WO wrote the manuscript. All of the authors study and authorized the final manuscript.FUNDINGThe study was funded by the National Council for Scientific and Technological Development (CNPq, Brazil, Grant 311281/20141), Minas Gerais Investigation Funding Foundation (FAPEMIG, Brazil, Grant APQ 00772-19), Coordination for the Improvement of Higher Education Personnel (CAPES, Brazil, Grants Finance Code 001 and PDSE-88881.132727/2016-01), and Cornell University’s College of Agriculture and Life Sciences.CONCLUSIONThe present study describes the assembly and functional annotation in the de novo transcriptome of M. glaucescens, a non-model plant with ornamental potential. This assembly was employed to study gene expression and determine genes associated with shoot organogenesis. The detection of many genes encoding putative transcription elements will benefit future research around the regulation of gene expression during the growth and improvement of M. glaucescens. Altogether, this study expanded the N-type calcium channel custom synthesis existing genomic sources for cacti and supplied a new platform for future genetic and biotechnological improvement of those plants for a variety of industrial purposes.ACKNOWLEDGMENTSThe authors would like to thank the funding bodies for their economic assistance. We also thank Delmar Lopes Alvim (in memoriam) for enable throughout fieldwork, Evandro S. B. Oliveira and Jessde Andrade Ribeiro for fruitful discussions on acupuncture in plants, the Boyce Thompson Institute (Ithaca, NY, USA) for delivering the server to carry out de novo transcriptome assembly, plus the Specht Lab (Cornell University) for providing the facilities to perform differential expression evaluation. Editage (http://editage) is acknowledged for the English language editing of this manuscript.Data AVAILABILITY STATEMENTThe information presented within the study are deposited in the NCBI Entrez repository (ncbi.nlm.nih.gov/biop