Or car. In either case C60 exposure exacerbated myocardial infarction. Inside every single delivery route,

Or car. In either case C60 exposure exacerbated myocardial infarction. Inside every single delivery route, infarct sizes inside the male groups have been larger than these in females. Among delivery routes, the females had larger infarctions in response to IT C60 exposure compared with IV exposure. p 0.05 by two-way ANOVA, N = 4?.mAChR4 Modulator Source exposed to IV C60 when compared with serum collected from male rats exposed to IV automobile and male rats exposed to IT C60 (Fig. 4A). Female rats exposed to IV C60 had significantly lower serum IL-6 concentrations than IV C60 exposed males. Serum IL-6 concentrations have been NPY Y1 receptor Antagonist list unaltered between all IT groups and IV exposed females. MCP-1 showed a comparable profile to IL-6. MCP-1 concentrations have been higher in serum-collected post-I/R from male rats exposed to C60 IV when compared with serum collected from male rats exposed to IV automobile, male rats exposed to IT C60 and female rats exposed to C60 IV (Fig. 4B). Female rats exposed to IV C60 had considerably decrease serum MCP1 concentrations than IV C60 exposed males. Serum MCP-1 concentrations had been unaltered amongst all IT groups and IV exposed females. VEGF was variable and slightly elevated in females exposed to IT and IV C60 , even though not substantially dif-TABLE two Rat Pulmonary Responses to Intratracheal (IT) or Intravenous (IV) Delivery of C60 or Car SuspensionsTotal bronchoalveolar lavage fluid (BALF) protein and cellularity BAL samples collected 24 h following exposure in males, information expressed as mean ?SEM Protein ( g/ml) IT Automobile 560 ?149 C60 316 ?55 Na�ve i 456 ?31 n four? p 0.05 versus IT C60. IV 638 ?one hundred 750 ?138 Cell number (?105 ) IT 5.7 ?1.four three.9 ?0.8 five.6 ?0.five four? IV 3.5 ?0.9 6.9 ?1.7 Macrophages (?105 ) IT five.three ?1.three 4.0 ?0.7 five.six ?0.5 three? IV five.7 ?0.7 2.5 ?1.2 Neutrophils (x 103 ) IT 14.2 ?10 13.3 ?12 7.7 ?4.two 3? IV two.1 ?0.three 1.7 ?1.four Eosinophils (?03 ) IT 1.9 ?1.1 0.5 ?0.5 1 3? IV 2.four ?two.four 1 Epithelial cells (?103 ) IT 6.6 ?2.four 2.8 ?0.3 eight.7 ?4.0 3? IV 1.7 ?1.7 8.two ?2.six CARDIOVASCULAR INJURY IN RESPONSE TO CTotal bronchoalveolar lavage fluid (BALF) protein and cellularity BAL samples collected 24 h following exposure in females, information expressed as mean ?SEM Protein ( g/ml) Automobile C60 Na�ve i n IT 252 ?28 206 ?24 IV 195 ?8.two 226 ?41 N/A 5? Cell quantity (?105 ) IT IV 7.8 ?1.9 4.1 ?0.6 7.1 ?1.2 3.five ?0.5 six.six ?1.0 four? Macrophages (?105 ) IT IV 1.six ?0.9 3.3 ?0.four 0.9 ?0.three three.1 ?1.1 6.6 ?1.1, four? Neutrophils (?103 ) IT 35.0 ?25.three 27.5 ?11.1 IV 1 1 Eosinophils (?103 ) IT IV 0.25 ?0.25 1.7 ?1.7 12.five ?7.five three.six ?0.6 1 four? Epithelial cells (?103 ) IT IV 132 ?90 346 ?132 75.0 ?33 362 ?59 179 ?43 four?1 four?N/A, not applicable. p 0.05 versus IT C60 . p 0.05 versus IT car.THOMPSON ET AL.TABLE 3 Rat Coronary Artery Pharmacological Responses for Serotonin (5-HT), ACh, SNP, and ET-Male rat EC50 s [nM] (mean ?SEM) IT delivery C60 5-HT ACh SNP ET-1 559 ?104 265 ?45 116 ?24 38 ?20 Vehicle 871 ?130 239 ?34 76 ?18 48 ?15 IT delivery 5-HT ACh SNP ET-1 C60 1.7 ?0.1 two.6 ?0.4 2.four ?0.six 1.3 ?0.two Vehicle 3.two ?0.7 1.7 ?0.1 2.2 ?0.three 1.0 ?0.05 IT delivery 5-HT ACh SNP ET-1 C60 813 ?128 1419 ?843 19 ?3 29 ?20 Car 1268 ?497 139 ?28 21 ?four six.5 ?two.4 IT delivery 5-HT ACh SNP ET-1 C60 1.4 ?0.four 1.0 ?0.four 1.6 ?0.three 1.four ?0.three Vehicle 1.0 ?0.2 1.six ?0.3 two.1 ?0.4 1.1 ?0.three p-value, n 0.39, 5 0.29, five 0.35, four 0.41, five C60 1.three ?0.four 1.0 ?0.2 2.0 ?0.three 1.1 ?0.two p-value, n C60 0.ten, 4 2.0 ?0.2 0.ten, 4 three.eight ?0.8 0.77, 4 2.1 ?0.three 0.09, six 1.1 ?0.1 Female rat EC50 s [nM] (imply ?SEM) p-value, n 0.06, four 0.33, four 0.13, 4 0.49, six C60 IV deliv.