, we selected representative active ingredients containing GRg1, GRe, GRb1, CCM, aurantio-obtusin-6-O-b-D-glucoside (AG), isorhamnetin-3-O-neohespeidoside (IN), TEO and HPD of YQJZG as the marker elements for the content material determination. This offers a methodological basis for the high-quality manage of YQJZG. Nevertheless, the application with the ultraviolet detector inside the determination of saponins is exceptionally restricted for the reason that of a lack of lengthy conjugated systems in their structures. In addition to, ginsenosides are low in content material and are susceptible to interference by other components in this formula. Though we purified and enriched it making use of different ways, the needs of content determination making use of the HPLC-PDA can’t be met. In recent years, UPLC-MS/MS has developed rapidly and has been widely made use of in the research field of TCMs. It has the positive aspects of effective separation capability, high sensitivity and specificity for quantitation, which can be in particular suitable for the determination of multicomponents in Chinese medicine formulae (Wang et al., 2014; Wang et al., 2012; Yi et al., 2019). Based on the above information, a fully validated UPLC-MS/MS technique was developed for the determination of multi elements in YQJZG. Then, this new process was successfully utilized for the content material determination of 3 batches of YQJZG developed in thelaboratory. This method greatly simplified the earlier enrichment and impurities removal steps. What’s far more, this developed method provides certain reference significance for the determination of elements with weak ultraviolet absorption, low content material and conveniently interfered by other impurities in other TCM formulae.2. Components and solutions two.1. Reagents and materials Both acetonitrile and methanol (HPLC grade) had been bought from Fisher Scientific (Fair Lawn, NJ, USA). Other chemical reagents had been analytical grade and have been purchased from Beihua Fine Chemical compounds Co., Ltd. (Beijing, China). CCM, HPD, GRg1, GRe, GRb1, AG, IN and TEO (Fig. 1) have been bought from National Institutes for Meals and Drug Manage (NFDC) (Beijing, China). The purity for every reference compound was 98 . The following manage TCM slices have been also bought from National Institutes for Food and Drug Handle (NFDC) (Beijing, China), such as Ginseng Radix et Rhizoma, Curcumae Longae Rhizoma, Citri Reticulatae Pericarpium, Cassiae Semen, Typhae Pollen. All TCMs (Ginseng Radix et Rhizoma, Curcumae Longae Rhizoma, Citri Reticulatae Pericarpium, Typhae Pollen, Cassiae Semen, Nelumbinis Folium, Crataegi Fructus, Atractylodis Macrocephalae Rhizoma, Alismatis Rhizoma and Laminariae Thallus Eckloniae Thallus) applied in this experiment were purchased from HancaotangFig.TRAT1 Protein supplier 1.Collagen alpha-1(VIII) chain/COL8A1 Protein MedChemExpress Chemical structures of eight marker elements in Yiqi Jiangzhi Granules: 1 GRg1; 2 GRe; three GRb1; four TEO; 5 IN; 6 HPD; 7 AG; eight CCM.PMID:28322188 S. Guo, S. Hu, L. Jiang et al.Chinese Herbal Medicines 14 (2022) 324Pharmaceutical Co., Ltd. (Hebei, China). 3 batches of YQJZG were produced in the laboratory. Synergy UV water purification program (Millipore, Bedford, MA, USA) and SARTORIUS-BS124S electronic analytical balance (Sartorius, Goettingen, Reduced Saxony, Germany) have been created to supply ultrapure water and weigh supplies, respectively. 2.2. Preparation of regular solutions Reference substances of GRg1, GRe, GRb1, AG, IN, CCM, TEO and HPD were weighed precisely and dissolved into methanol to prepare the mixed stock options of reference standards with the concentrations of 109.0 lg/mL, 198.0 lg.
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