GST Nanoselector Agarose

Description:
| Description: GST Nanoselector Agarose have been specifically designed to bind GST-fusion proteins. GST Nanoselector Agarose is based on small high-affinity recombinant alpaca antibody fragments covalently coupled to the surface of Agarose. GST Nanoselector Agarose is an ideal tool to isolate or purify GST-fusion proteins fast and efficiently. | Ligand: Anti-GST single domain antibody fragment (VHH, Nanobody) | Bead size: ~ 40µm | Reactivity: Recognizes GST or GST fusion protein. | Binding capacity: High binding capacity, 10 µL slurry bind about 20 µg of recombinant GST. | Storage: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for 1 year. Do not freeze. | Storage | Buffer: 50 % slurry in PBS containing 20 % Ethanol | Background:Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. GST can be added to a protein of interest to purify it from solution in a process known as a pull-down assay. This is accomplished by inserting the GST DNA coding sequence next to that which codes for the protein of interest. Thus, after transcription and translation, the GST protein and the protein of interest will be expressed together as a fusion protein. GST Nanoselector Agarose is a ready to use resin for immunoprecipitation/pulldown of GST fusion proteins.