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Leptin (rat), ELISA kit
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No edge effect – all incubations at room temperature eliminate risk of evaporation at 37°C Broad dynamic range – easily quantify high and low levels of leptin Convenient- store all reagents at 4°C Fast – fully quantitative results in just 3 hours from up to 39 samples in duplicate Pre-coated plates and ready-to-use reagents save you time The Leptin (rat), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 3 hours.The adipokine leptin is a hormone secreted predominantly by adipose tissue that signals through leptin receptors in the hypothalamus to decrease appetite and increase energy expenditure. Binding of leptin to the long-form of the leptin receptor in the hypothalamus reduces neuropeptide Y (NPY) and agouti-related protein (AgRP) activity, while stimulating anorexigenic pro-opiomelanocortin (POMC) neuron activity to reduce appetite. In peripheral tissues, leptin antagonizes insulin signaling, increases fatty acid oxidation, decreases insulin production in pancreatic beta cells, and promotes fertility. Its expression in adipocytes can be regulated in a paracrine fashion by other adipokines such as IL-6 (stimulation) and TNF-α (inhibition). Leptin deficient mice display severe insulin resistance, obesity, and decreased fertility, all of which are reversible by administration of exogenous leptin. In contrast, elevated circulating levels of leptin are associated with increased obesity, indicative of an acclimated state of leptin resistance which is not well understood. Leptin (rat), ELISA kit Kit graph Leptin (rat), ELISA kit Kit graph
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| Alternative Name OB gene product | Application Colorimetric detection, ELISA | Application Notes For the quantitative determination of rat leptin in culture supernatants, plasma, and serum.{{2600559-12-2} MedChemExpress|{2600559-12-2} Technical Information|{2600559-12-2} Formula|{2600559-12-2} custom synthesis} | Assay Time 3 hours | Contents Microtiter plate, Standard, Antibody, Conjugate, Assay buffer 28, TMB Substrate, Stop solution 2, Wash buffer concentrate | Sensitivity 67.{{1834513-65-3} site|{1834513-65-3} Protocol|{1834513-65-3} Data Sheet|{1834513-65-3} supplier} 2 pg/ml (range 100 – 6,400 pg/ml) | Species Reactivity Rat | Technical Info / Product Notes Cited samples:Cytokine ELISAs Cited Samples | UniProt ID P50596 | Wavelength 450 nm