Product Name :
[pThr1342]DNA-topoisomerase II α monoclonal antibody (3D4)
Sequence:
Purity:
Molecular Weight:
Solubility :
Appearance:
Use/Stability :
Description:
DNA-topoisomerase II, also known as Topo II or Top 2, is a multifunctional nuclear enzyme required during DNA replication, chromosome disjunction at mitosis, and other DNA-related activities because of its ability to alter DNA supercoiling. In mammalian cells, Topo II consists of two isozymes, Topo II-alpha (170kDa) and Topo II-beta (180kDa) and expression and localization of each isoform are distinct and stage specific during the cell cycle. Topo II-beta is expressed constitutively throughout the cell cycle, whereas the expression of Topo II-alpha is cell cycle-regulated, peaking in G2 to M phase and declining to a minimal level at the end of M phase. DNA-topoisomerase II is the target of some of the most active drugs used in chemotherapy and has been shown to have a critical role in neural development. Western blot analysis of Jurkat cells using DNA-Topoisomerase IIa (pThr1342) mAb (3D4). Immunocytochemistry analysis of human HEp-2 cells stained with DNA-Topoisomerase IIa (pThr1342) mAb (3D4).{{1268524-70-4} site|{1268524-70-4} Protocol|{1268524-70-4} In Vitro|{1268524-70-4} custom synthesis} Western blot analysis of Jurkat cells using DNA-Topoisomerase IIa (pThr1342) mAb (3D4). Immunocytochemistry analysis of human HEp-2 cells stained with DNA-Topoisomerase IIa (pThr1342) mAb (3D4).
CAS :
Solubility:
Formula:
Additional Information :
| Application ICC, WB | Application Notes Detects a band of ~170kDa by Western blot.{{2765082-12-8} site|{2765082-12-8} Technical Information|{2765082-12-8} Purity|{2765082-12-8} custom synthesis} | Clone 3D4 | Formulation Liquid.PMID:29083568 In PBS, pH 7.2, containing 50% glycerol. | Host Mouse | Immunogen Synthetic peptide corresponding to a portion of human Topoisomerase IIα phosphorylated at Tyr1342. | Isotype IgG1 | Recommendation Dilutions/Conditions Immunocytochemistry (10µg/ml)Western Blot (1-10µg/ml, colorimetric)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. | Species Reactivity Human | UniProt ID P11388 (α isozyme), Q02880 (β isozyme) | Unit of Measure (UM) µg
