Limit the ongoing response in order to protect the host from

Limit the ongoing response in order to protect the host from excessive immune mediated tissue destruction (reviewed in [4]), which is one of the characteristics in RA. Support for a role of IL-10 in RA comes from mouse models: in the CIA model, treatment with antiDisease-Dependent IL-10 Ameliorates CIAIL-10 antibodies aggravates the disease, as does a complete lack of IL-10 [5,6]. This argues for IL-10 as a possible cytokine to use for treatment of RA. Indeed, addition of recombinant IL-10 [7], transfer of IL-10 producing cells [8] or continuous Title Loaded From File production of IL-10 [9,10,11], reduces the severity but not the frequency of CIA. However, a permanent increase in IL-10 levels may not be optimal as it may also influence defence towards invading pathogens whereas an increase exclusively during inflammation (flares) would be preferable and could provide a treatment alternative in CIA and RA. Inflammation induced IL-10 transcription in endothelial cells, driven by an E selectin promoter, has been used by Garaulet et al. and showed promising results in ameliorating arthritis [12]. We sought to investigate whether IL-10 expression induced by a promoter sensitive to pro-inflammatory cytokines IL-6 and IL1 in haematopoetic cells, could be a candidate for tailor-made therapy for CIA and with a long term goal also for RA patients. Our data show that inflammation-induced local expression of IL10 delays progression of CIA through decreased serum levels of IL-6 and anti-CII antibodies. This study provides evidence that inflammation-dependent immunosuppression is a promising tool for the treatment of autoimmune arthritis.groups 1480666 (Figure 2 D ). Analysing IL-10 in serum by ELISA showed similar levels in both groups of mice (data not shown). Taken together this suggests that IL-10 acts locally in the lymph nodes rather than on a systemic level. To investigate the link between increased IL-10 production and suppression of arthritis we determined the mRNA levels of the suppressors of cytokine signalling 1 and 3 (SOCS1 and SOCS3). The SOCS proteins are key negative regulators of cytokine responses and act via inhibition of the intracellular JAK/STAT signalling pathways [14], and IL-10 has previously been shown to induce these adaptor proteins [15]. We found elevated mRNA levels of SOCS1 and the same tendency (p = 0.12) also for SOCS3 in peripheral lymph nodes in LNT-IL-10 mice (Figure 2G). These data show that a local increase in IL-10 results in an increase in SOCS expression which Title Loaded From File correlates with suppression of arthritis development.LNT-IL-10 Influences Serum Protein Levels 1407003 of Cytokines and Anti-CII AntibodiesThe effect by IL-10 may be direct or indirect and we were, therefore, interested in potential effects on other cytokines. Indeed, we found a significant decrease in serum levels of IL-6 in LNT-IL10 mice at day 29 after CII immunisation (Figure 3A). At day 42, although the levels were still very low in LNT-IL-10 mice, the levels of IL-6 in control mice had declined and the difference between the groups were no longer significant. Serum levels of a number of additional cytokines (IL-1a, IL-2, IL-4, IL-5, IL-10, IL-13, IL-17A, IL-21, IL-27, IFN-c) were measured without any significant differences between the groups (data not shown). Previous work have shown that IL-6 promotes the development of arthritis as it together with TGF-b induces Th17 cells and stimulates B cells to increased production of IgG and IgA antibodies [16]. As may be expected, based on it.Limit the ongoing response in order to protect the host from excessive immune mediated tissue destruction (reviewed in [4]), which is one of the characteristics in RA. Support for a role of IL-10 in RA comes from mouse models: in the CIA model, treatment with antiDisease-Dependent IL-10 Ameliorates CIAIL-10 antibodies aggravates the disease, as does a complete lack of IL-10 [5,6]. This argues for IL-10 as a possible cytokine to use for treatment of RA. Indeed, addition of recombinant IL-10 [7], transfer of IL-10 producing cells [8] or continuous production of IL-10 [9,10,11], reduces the severity but not the frequency of CIA. However, a permanent increase in IL-10 levels may not be optimal as it may also influence defence towards invading pathogens whereas an increase exclusively during inflammation (flares) would be preferable and could provide a treatment alternative in CIA and RA. Inflammation induced IL-10 transcription in endothelial cells, driven by an E selectin promoter, has been used by Garaulet et al. and showed promising results in ameliorating arthritis [12]. We sought to investigate whether IL-10 expression induced by a promoter sensitive to pro-inflammatory cytokines IL-6 and IL1 in haematopoetic cells, could be a candidate for tailor-made therapy for CIA and with a long term goal also for RA patients. Our data show that inflammation-induced local expression of IL10 delays progression of CIA through decreased serum levels of IL-6 and anti-CII antibodies. This study provides evidence that inflammation-dependent immunosuppression is a promising tool for the treatment of autoimmune arthritis.groups 1480666 (Figure 2 D ). Analysing IL-10 in serum by ELISA showed similar levels in both groups of mice (data not shown). Taken together this suggests that IL-10 acts locally in the lymph nodes rather than on a systemic level. To investigate the link between increased IL-10 production and suppression of arthritis we determined the mRNA levels of the suppressors of cytokine signalling 1 and 3 (SOCS1 and SOCS3). The SOCS proteins are key negative regulators of cytokine responses and act via inhibition of the intracellular JAK/STAT signalling pathways [14], and IL-10 has previously been shown to induce these adaptor proteins [15]. We found elevated mRNA levels of SOCS1 and the same tendency (p = 0.12) also for SOCS3 in peripheral lymph nodes in LNT-IL-10 mice (Figure 2G). These data show that a local increase in IL-10 results in an increase in SOCS expression which correlates with suppression of arthritis development.LNT-IL-10 Influences Serum Protein Levels 1407003 of Cytokines and Anti-CII AntibodiesThe effect by IL-10 may be direct or indirect and we were, therefore, interested in potential effects on other cytokines. Indeed, we found a significant decrease in serum levels of IL-6 in LNT-IL10 mice at day 29 after CII immunisation (Figure 3A). At day 42, although the levels were still very low in LNT-IL-10 mice, the levels of IL-6 in control mice had declined and the difference between the groups were no longer significant. Serum levels of a number of additional cytokines (IL-1a, IL-2, IL-4, IL-5, IL-10, IL-13, IL-17A, IL-21, IL-27, IFN-c) were measured without any significant differences between the groups (data not shown). Previous work have shown that IL-6 promotes the development of arthritis as it together with TGF-b induces Th17 cells and stimulates B cells to increased production of IgG and IgA antibodies [16]. As may be expected, based on it.