K487:SMC2K417+K781 K480:SMC2K417+K781 SMC4K473+K854 K779 SMC4K480+K487:K781 SMC2K789 +K797 SMC4K850 +K852 K858 14.4?19.0?SMC4K478 +K480 K473 SMC4K854 K865 KSMC2KK792:SMC4KK797 K802:SMC4K458 K458:SMC2K792 +K802 possible short loop/disruption E447-K456 29.4?6.5?K919 K925:SMC2K348 K336:SMC4K943 K332:SMC4K943 180?K863 18.2?K869 K943:SMC2K332+K336 K321:SMC4K396 K869:SMC4K396 K312:SMC4K400 +K402+K405 SMC4K426:K419 K413 K405:SMC2K312 SMC4K943:K953 K402:SMC2K312 K400:SMC2K312 K396:SMC2K321 possible short loop/disruption +K869 L379-N384 7.1?K375 K371 K367:SMC2K887 +K890 SMC2 hinge SMC4 heads 18.5?180?K1006:SMC2K275+K898 12.8?K367 K364 K362 K360 8.1?18.8?K346 K1012 17.7?K354 K351 18.2?K1006 32.7?K338 16.9?K458 19.6?K450 33.8?180?K919 K925 14.8?K932 K943 K456 24.5?K902 30.9?K448 Kpossible short loop/disruption K830-Q839 K350 14.3?K343 21.6?K332 20.9?K11.4?K356 K354 6.7?K350 K348:SMC4KK448 KK887 16.7?SMC2K272:KK887:SMC4K367 K890:SMC4K367 SMC2K15.6?Figure 7. Some of the building blocks used to assemble the central portion of the condensin anti-parallel coiled-coils. Five of the 10 coiled-coil fragments modelled in this study are shown in two views each, providing full annotation detail of intra- and interdomain cross-links (red brackets with Xwalk SAS distances if both lysines are on the same fragment). Intermolecular cross-links are specified in the inner panel images from residues numbered in red font. These fragments span the central portion of the coiled-coil and include two sites with multiple intermolecular links (see also figure 8c). Their location in the three-dimensional model is shown schematically in the overview schematic (SMC2 residue ranges 395?469 ?746?786 (top), 293?386 ?792?895 (bottom); SMC4 residue ranges 479 ??544 ?793?845 (top), 431?477 ?855?945 (middle), 342?421 ?949?1034 (bottom). Images produced with PYMOL v. 1.7 (Schrodinger, LLC).(a)rsob.royalsocietypublishing.org(b)Open Biol. 5:(c)10 nm(d ) no. amino acids in gap 10 8 6 4 2 0 0 1 2 3 4 Sulfatinib cancer average distance between amino acids in gap (Ca) (? number (e) 40 30 20 105 10 15 20 25 30 Ca ?Ca distances (105 measurable cross-links)Figure 8. Low-resolution approximation of the three-dimensional structure of the SMC2/SMC4 core of chicken condensin generated through template-assisted rigid assembly of 13 fragments. (a) Ribbon depiction of the 1096 SMC2 residues (92 ) and 1111 SMC4 residues (85 ) included in the model. Orange and red spheres depict Lys a found in at least one high-confidence cross-link (grey spheres are unlinked lysines). Arrows mark where four sites on SMC2 and SMC4 predicted as possibly irregular in 2002 (loops I and III according to Beasley et al. [43]) line up on the modelled dimer although helical fragments were assembled solely based on the cross-linking data. (b) All-atom depiction of the model. Black lines denote the intramolecular links found between `domains’ (table 1), which includes those between the anti-parallel helices in the coiled-coils that we used to derive/confirm their approximate relative alignments in each modelled fragment. The Ca a distance average across these interdomain intramolecular cross-links ??(nine in SMC2; 12 in SMC4) was 16 + 5.9 A. The X-walk SAS Cb-distance average over the 16 in-fragment cross-links among them was 18 + 5.7 A. For NS-018 structure comparison, the ?and the X-walk SAS Cb-distance average over the 53 in-fragment cross-links among Ca a distance average of the 57 intradomain cross-links (not shown) was 12 + 4.6 A ?them was 16 + 7.3 A.K487:SMC2K417+K781 K480:SMC2K417+K781 SMC4K473+K854 K779 SMC4K480+K487:K781 SMC2K789 +K797 SMC4K850 +K852 K858 14.4?19.0?SMC4K478 +K480 K473 SMC4K854 K865 KSMC2KK792:SMC4KK797 K802:SMC4K458 K458:SMC2K792 +K802 possible short loop/disruption E447-K456 29.4?6.5?K919 K925:SMC2K348 K336:SMC4K943 K332:SMC4K943 180?K863 18.2?K869 K943:SMC2K332+K336 K321:SMC4K396 K869:SMC4K396 K312:SMC4K400 +K402+K405 SMC4K426:K419 K413 K405:SMC2K312 SMC4K943:K953 K402:SMC2K312 K400:SMC2K312 K396:SMC2K321 possible short loop/disruption +K869 L379-N384 7.1?K375 K371 K367:SMC2K887 +K890 SMC2 hinge SMC4 heads 18.5?180?K1006:SMC2K275+K898 12.8?K367 K364 K362 K360 8.1?18.8?K346 K1012 17.7?K354 K351 18.2?K1006 32.7?K338 16.9?K458 19.6?K450 33.8?180?K919 K925 14.8?K932 K943 K456 24.5?K902 30.9?K448 Kpossible short loop/disruption K830-Q839 K350 14.3?K343 21.6?K332 20.9?K11.4?K356 K354 6.7?K350 K348:SMC4KK448 KK887 16.7?SMC2K272:KK887:SMC4K367 K890:SMC4K367 SMC2K15.6?Figure 7. Some of the building blocks used to assemble the central portion of the condensin anti-parallel coiled-coils. Five of the 10 coiled-coil fragments modelled in this study are shown in two views each, providing full annotation detail of intra- and interdomain cross-links (red brackets with Xwalk SAS distances if both lysines are on the same fragment). Intermolecular cross-links are specified in the inner panel images from residues numbered in red font. These fragments span the central portion of the coiled-coil and include two sites with multiple intermolecular links (see also figure 8c). Their location in the three-dimensional model is shown schematically in the overview schematic (SMC2 residue ranges 395?469 ?746?786 (top), 293?386 ?792?895 (bottom); SMC4 residue ranges 479 ??544 ?793?845 (top), 431?477 ?855?945 (middle), 342?421 ?949?1034 (bottom). Images produced with PYMOL v. 1.7 (Schrodinger, LLC).(a)rsob.royalsocietypublishing.org(b)Open Biol. 5:(c)10 nm(d ) no. amino acids in gap 10 8 6 4 2 0 0 1 2 3 4 average distance between amino acids in gap (Ca) (? number (e) 40 30 20 105 10 15 20 25 30 Ca ?Ca distances (105 measurable cross-links)Figure 8. Low-resolution approximation of the three-dimensional structure of the SMC2/SMC4 core of chicken condensin generated through template-assisted rigid assembly of 13 fragments. (a) Ribbon depiction of the 1096 SMC2 residues (92 ) and 1111 SMC4 residues (85 ) included in the model. Orange and red spheres depict Lys a found in at least one high-confidence cross-link (grey spheres are unlinked lysines). Arrows mark where four sites on SMC2 and SMC4 predicted as possibly irregular in 2002 (loops I and III according to Beasley et al. [43]) line up on the modelled dimer although helical fragments were assembled solely based on the cross-linking data. (b) All-atom depiction of the model. Black lines denote the intramolecular links found between `domains’ (table 1), which includes those between the anti-parallel helices in the coiled-coils that we used to derive/confirm their approximate relative alignments in each modelled fragment. The Ca a distance average across these interdomain intramolecular cross-links ??(nine in SMC2; 12 in SMC4) was 16 + 5.9 A. The X-walk SAS Cb-distance average over the 16 in-fragment cross-links among them was 18 + 5.7 A. For comparison, the ?and the X-walk SAS Cb-distance average over the 53 in-fragment cross-links among Ca a distance average of the 57 intradomain cross-links (not shown) was 12 + 4.6 A ?them was 16 + 7.3 A.
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BET Bromodomain Inhibitor
